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Diptera.info :: General Diptera forums :: Methodology
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Advice on Barcoding museum specimens of Diptera
peterorrell
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Posted on 29-03-2016 18:52
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Hello,

I am currently completing my PhD, which looks into how altering the belowground mycorrhizal community has the potential to influence pollinator communities in strawberry plants. Over the past two years I have collected a number of pollinators visiting my plants, and I am now in the process of identifying them all. I am confident taxonomically identifying hymenoptera and hoverflies, however I have been advised that taxonomic ID of the remainder of the Diptera is rather difficult, and that I am better off identifying them through barcoding. So far I have tested a NaOH extraction method, along with a separate Proteinase K extraction method, however both haven't been very successful, and I am seeing a success rate of less than 40%. I think that this may be due to the fact that the some of the samples were pinned this time last year, and some over the last month, and in turn the fact that they have now dried, which is causing these issues.

I was wondering if anyone has experience in barcoding museum specimens of Diptera/insects that have been dried, and would be able to provide any advice on the methods that you use? In an ideal world I would prefer to just extract a leg from each sample if possible, in order to keep the remainder of the sample for a reference collection.

Thank you very much ahead of time, and I hope you have a nice evening!
 
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01.03.21 06:08
How do I submit photos for identification

23.02.21 10:02
Probably something in the file's 'ource data. I edited it and saved as png. Now it is okay.

22.02.21 14:13
What happened to the picture in https://diptera.in
fo/forum/viewthrea
d.php?thread_id=99
500 ? It had been comressed only horizontally...

20.02.21 22:13
Markus Gebel

20.02.21 18:12
@beateSenf you can check the coincident threads with your species and try to write to whom identified them

11.02.21 09:15
No worries. Not everyone has time all the time. Wink

06.02.21 22:18
I am sincerely sorry for this comment. You are doing your best and I am very grateful for your work. I'm sorry. Beate

06.02.21 18:23
Hallo, I have a question. Are there any hidden rules in this forum? Do you have to upload spectacular flies to get answers? As soon as the inquiries slide to page two or further, they rest gently

21.01.21 08:58
No idea what happened but it is corrected

11.01.21 22:32
my portrait in portrait format is squashed into landscape format. how can I prevent that next time? Greetings Beate

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