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Diptera.info :: Miscellaneous :: General queries
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A question about Fleas (Siphonaptera)
pwalter
#1 Print Post
Posted on 09-06-2009 13:21
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Location: Miskolc, Hungary
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HI! I finally managed to catch a flea of a dog - first time in my life I see this insect order. Can anyone suggest how to examine it? Should I use NaOH to make it transparent with microscope? Should I dissect genitalia? How? It's some 2,5 mm, so not too small.
 
phil withers
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Posted on 09-06-2009 14:07
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Location: Lyon, France
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Clear with NaOH then mount in DMSO or on a slide in...whatever you like. You cannot dissect the genitalia, they are internal.
 
pwalter
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Posted on 09-06-2009 14:23
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Thank You!
 
Paul Beuk
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Posted on 09-06-2009 14:35
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DMSO?
Paul

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Tony Irwin
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Posted on 09-06-2009 15:55
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Dimethyl sulfoxide
Tony
----------
Tony Irwin
 
pwalter
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Posted on 09-06-2009 16:02
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I was suprised to hear DMSO - at the university we use it for cryoprotection of mammalian cells stored in liquid nitrogen.
 
phil withers
#7 Print Post
Posted on 09-06-2009 21:24
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Location: Lyon, France
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I meant DMHF - sorry (dimethyl hydantoin formaldehyde, before Paul asks). DMSO just slipped out...must have been thinking of my old life there...
 
Paul Beuk
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Posted on 10-06-2009 08:33
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Is it a mounting agent or something to store it in?
Paul

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phil withers
#9 Print Post
Posted on 10-06-2009 09:03
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Location: Lyon, France
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Mounting agent (I use it for genitalia...not mine though). It is crystalline, and when added to water forms a clear liquid which dries to transparent: the meniscus formed helps by magnifying too.
 
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