Thread subject: Diptera.info :: Storing flies in alcohol

Posted by Adrian on 12-11-2007 16:12
#46

Agreed. Wings can be a problem with some flies. I dry mine by washing first in 100% ethanol and then 24 h in ethyl acetate. I then pick up the .wet. fly from the EtAc using very fine forceps and place it carefully on fclean fine grade filter paper (using ventilation as the EtAc isn't good for you). As I place it on the paper, I make sure that the tip of the lower wing contacts first after which the rest of the wing usually snaps flat onto the paper (surface tension effect). I then release the insect so that it falls onto the paper. Usually the other wing will remain reasonably flat but if it does not, a very fine pin can be used to insert into any folds to gently lever them apart.
You will always have problems if you dry directly from alcohol:- firstly, they are not really dry as ethanol is not a good dehydrating agent. Secondly, it evapourates too slowly and surface tension of the liquid is much higher than with EtAc and will ensure that any fold with liquid trapped in between will stay folded. The evapouration rate of EtAc is so rapid that there is a slight gassing out effect as it dries:- tending to force apart folds in the wing membrane.
If you have the kit, critical point drying tends to leave wings fairly well extended. Its quite good (but expensive) but I only use is for drying bulk samples where I havn't the time to spend preparing every specimen with extreme care.
Hope this helps
cheers
Adrian