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Hi!

Unfortunately genome sequencing requires a lot more DNA than for PCR/Sanger for COI etc (which need <1ng DNA). Minimum for Novaseq/short-read genome might be ~20ng at ~2ng/ul, and in fact we often struggle to get enough at sufficient concentration from single Drosophila.

But this would actually be going for ONT long read, which really would benefit from the whole fly. For comparison, Darwin tree of life ask for 4 specimens when doing stuff this small (Hifi,HiC,RNA, and spare!)

It's part of a Drosophilidae project, but the world is desperately short of suitable outgroup genomes (i.e. Ephydroidea) and so my collaborator is searching for (fresh, -20C in Ethanol) specimens to sequence!

The species really don't matter, so long as they sit between Opomyzoidea/Carnoidea and Drosophilidae!

I'll check with my collaborator, and see what he thinks.

Thanks!

D