Thread subject: Diptera.info :: How to prepare diptera genitalia?

Posted by xylo on 06-12-2005 10:13
#2

Hi Andrius,

I do not know, if there is a best technique... I think it depends on the group you like to work on.... I am still working with Canada balsam to embed genitalia and the whole gnat... This might be not that healthy, because you need beside alcohol (70%-96%) xylene and creosote.... but it works fine and the dose makes the poison.... if you have the possibility to use an extraction hood or a room well ventilated.....
However, there are some other mounting media.... Hoyer's, PVA, PVL... may producing artefacts or destroying mounted specimens on slide..... Euparal... shrinkage and getting fast lacky....
Do you have already checked the special literature dealing with your nematoceran group?!? Probably, there are the hints you need....

You have the choice.... ;-)

so long,
xylo