Posted by John Carr on 07-07-2021 19:05
#6
Xespok wrote:
I am pretty sure that unless there is some water in the mixture, the problem of hardening of the flies due to dehydration is inevitable. If however there is water in the system the DNA will spontaneously hydrolyse over time to some extent. I am not sure if this is still enzyme mediated reaction or not.
If I understand you, I should use pure alcohol, rather than denatured one.
If water damages DNA but methanol, propanol, and similar organic compounds do not then denatured alcohol should work. I don't know if that is true. But beware that some chemicals will absorb water from the air.